Periodontitis marker bacteria analysis for patient-oriented therapy.

The iai PadoTest is a microbiological test that detects periodontitis-causing bacteria and recommends targeted treatment. Dentists and dental hygienists all over Europe have been successfully using the iai PadoTest for many years.

Bacterial detection with the iai PadoTest is essential for:

  • the correct choice of treatment
  • targeted medication without unnecessary treatment with antibiotics
  • quality assurance following treatment - proof of efficiency
  • long-term monitoring of treated cases
  • differential diagnosis

Periodontal disease is an infectious disease of the periodontium that is caused by specific bacteria. The type of bacterial association characterises the severity of periodontitis. The Iai PadoTest is used to determine the presence and association of periodontopathogenic germs and classify them into five statistically determined degrees of severity of periodontitis (types 1 to 5).

This typing allows therapists to initiate targeted treatment very easily and efficiently. The iai PadoTest provides information on treatment options and, if necessary, on necessary antibiotics.

The investigation focuses on periodontopathogenic germs:

  • Aggregatibacter actinomycetemcomitans (Aa)
  • Tannerella forsythia (Tf)
  • Porphyromonas gingivalis (Pg)
  • Treponema denticola (Td)
  • Porphyromonas intermedia (Pi)
  • Filifactor alocis (Fa)

The iai PadoTest uses a special Multiplex Realtime PCR (Polymerase Chain Reaction) for detection and quantification. The unsurpassed accuracy of this form of analysis provides an accurate picture of the bacterial status in the periodontal pocket. Our own investigations have shown that the detection of already dead bacteria is negligible, since the metabolism in the periodontal pocket is subject to a certain turn-over rate. Real-time PCR and probe-based RNA hybridisation were correlated with each other and the PCR results were confirmed using Next Generation Sequencing (NGS). Of course, all results from the RNA hybridisation remain valid and along with the correlation enable a seamless transition to real-time PCR as an examination method.

In terms of price, the iai PadoTest is extremely attractive and can be used very flexibly thanks to the availability of multi-site and single-station tests.

The therapist recognises the damage, the iai PadoTest finds the cause and then recommends the type of treatment!

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